Heterologous expression of manganese peroxidase in Aspergillus niger and its effect on phenanthrene removal from soil.
Identifieur interne : 000504 ( Main/Exploration ); précédent : 000503; suivant : 000505Heterologous expression of manganese peroxidase in Aspergillus niger and its effect on phenanthrene removal from soil.
Auteurs : Diana V. Cortés-Espinosa [Mexique] ; Ángel E. Absal N ; Noé Sanchez ; Octavio Loera ; Refugio Rodríguez-Vázquez ; Francisco J. FernándezSource :
- Journal of molecular microbiology and biotechnology [ 1660-2412 ] ; 2011.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Amorces ADN (génétique), Aspergillus niger (enzymologie), Aspergillus niger (génétique), Aspergillus niger (métabolisme), Biotransformation (MeSH), Facteurs temps (MeSH), Peroxidases (génétique), Peroxidases (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Phénanthrènes (métabolisme), Polluants du sol (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Réaction de polymérisation en chaîne (MeSH), Sélection génétique (MeSH), Transformation génétique (MeSH).
- MESH :
- enzymologie : Aspergillus niger, Phanerochaete.
- génétique : ADN fongique, Amorces ADN, Aspergillus niger, Peroxidases, Phanerochaete, Protéines recombinantes.
- métabolisme : Aspergillus niger, Peroxidases, Phénanthrènes, Polluants du sol, Protéines recombinantes.
- Biotransformation, Facteurs temps, Réaction de polymérisation en chaîne, Sélection génétique, Transformation génétique.
English descriptors
- KwdEn :
- Aspergillus niger (enzymology), Aspergillus niger (genetics), Aspergillus niger (metabolism), Biotransformation (MeSH), DNA Primers (genetics), DNA, Fungal (genetics), Peroxidases (genetics), Peroxidases (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Phenanthrenes (metabolism), Polymerase Chain Reaction (MeSH), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Selection, Genetic (MeSH), Soil Pollutants (metabolism), Time Factors (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA Primers, DNA, Fungal, Peroxidases, Recombinant Proteins.
- enzymology : Aspergillus niger, Phanerochaete.
- genetics : Aspergillus niger, Phanerochaete.
- metabolism : Aspergillus niger, Peroxidases, Phenanthrenes, Recombinant Proteins, Soil Pollutants.
- Biotransformation, Polymerase Chain Reaction, Selection, Genetic, Time Factors, Transformation, Genetic.
Abstract
A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.
DOI: 10.1159/000331563
PubMed: 22286039
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Aspergillus niger (genetics)</term>
<term>Aspergillus niger (metabolism)</term>
<term>Biotransformation (MeSH)</term>
<term>DNA Primers (genetics)</term>
<term>DNA, Fungal (genetics)</term>
<term>Peroxidases (genetics)</term>
<term>Peroxidases (metabolism)</term>
<term>Phanerochaete (enzymology)</term>
<term>Phanerochaete (genetics)</term>
<term>Phenanthrenes (metabolism)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Selection, Genetic (MeSH)</term>
<term>Soil Pollutants (metabolism)</term>
<term>Time Factors (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term>
<term>Amorces ADN (génétique)</term>
<term>Aspergillus niger (enzymologie)</term>
<term>Aspergillus niger (génétique)</term>
<term>Aspergillus niger (métabolisme)</term>
<term>Biotransformation (MeSH)</term>
<term>Facteurs temps (MeSH)</term>
<term>Peroxidases (génétique)</term>
<term>Peroxidases (métabolisme)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Phanerochaete (génétique)</term>
<term>Phénanthrènes (métabolisme)</term>
<term>Polluants du sol (métabolisme)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Réaction de polymérisation en chaîne (MeSH)</term>
<term>Sélection génétique (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term>
<term>DNA, Fungal</term>
<term>Peroxidases</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Aspergillus niger</term>
<term>Phanerochaete</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Aspergillus niger</term>
<term>Phanerochaete</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus niger</term>
<term>Phanerochaete</term>
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<term>Amorces ADN</term>
<term>Aspergillus niger</term>
<term>Peroxidases</term>
<term>Phanerochaete</term>
<term>Protéines recombinantes</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Aspergillus niger</term>
<term>Peroxidases</term>
<term>Phenanthrenes</term>
<term>Recombinant Proteins</term>
<term>Soil Pollutants</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aspergillus niger</term>
<term>Peroxidases</term>
<term>Phénanthrènes</term>
<term>Polluants du sol</term>
<term>Protéines recombinantes</term>
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<keywords scheme="MESH" xml:lang="en"><term>Biotransformation</term>
<term>Polymerase Chain Reaction</term>
<term>Selection, Genetic</term>
<term>Time Factors</term>
<term>Transformation, Genetic</term>
</keywords>
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<term>Facteurs temps</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sélection génétique</term>
<term>Transformation génétique</term>
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<front><div type="abstract" xml:lang="en">A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.</div>
</front>
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<Title>Journal of molecular microbiology and biotechnology</Title>
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<Abstract><AbstractText>A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.</AbstractText>
<CopyrightInformation>Copyright © 2012 S. Karger AG, Basel.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cortés-Espinosa</LastName>
<ForeName>Diana V</ForeName>
<Initials>DV</Initials>
<AffiliationInfo><Affiliation>Centro de Investigación en Biotecnologóa Aplicada del IPN, Carretera Federal Santa Inés, Tepetitla de Lardizabal, Mexico.</Affiliation>
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<Author ValidYN="Y"><LastName>Absalón</LastName>
<ForeName>Ángel E</ForeName>
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<Author ValidYN="Y"><LastName>Sanchez</LastName>
<ForeName>Noé</ForeName>
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<Author ValidYN="Y"><LastName>Loera</LastName>
<ForeName>Octavio</ForeName>
<Initials>O</Initials>
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<Author ValidYN="Y"><LastName>Rodríguez-Vázquez</LastName>
<ForeName>Refugio</ForeName>
<Initials>R</Initials>
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<Author ValidYN="Y"><LastName>Fernández</LastName>
<ForeName>Francisco J</ForeName>
<Initials>FJ</Initials>
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<MeshHeading><DescriptorName UI="D012641" MajorTopicYN="N">Selection, Genetic</DescriptorName>
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<MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName>
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<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
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<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
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<name sortKey="Loera, Octavio" sort="Loera, Octavio" uniqKey="Loera O" first="Octavio" last="Loera">Octavio Loera</name>
<name sortKey="Rodriguez Vazquez, Refugio" sort="Rodriguez Vazquez, Refugio" uniqKey="Rodriguez Vazquez R" first="Refugio" last="Rodríguez-Vázquez">Refugio Rodríguez-Vázquez</name>
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<country name="Mexique"><noRegion><name sortKey="Cortes Espinosa, Diana V" sort="Cortes Espinosa, Diana V" uniqKey="Cortes Espinosa D" first="Diana V" last="Cortés-Espinosa">Diana V. Cortés-Espinosa</name>
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